Tobias Heinks
Profile
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Dr. Tobias Heinks |
Tobias Heinks on ORCiD
Curriculum Vitae (Short Version)
- 10.2023+ Postdoctoral Scientist Otto-von-Guericke University, Biocatalysis
- 10.2019 - 07.2023 Doctor of Natural Sciences (Dr. rer. nat) University of Bielefeld, Bielefeld, Biochemistry
Doctoral thesis: Cascading and immobilization of amine transaminases for the biosynthesis of enantiomerically pure amines
Degree: Summa Cum Laude
Supervisor: Prof. Dr. Gabriele Fischer von Mollard
Co-supervisors: Prof. Dr. Uwe Bornscheuer, Prof. Dr. Matthias Höhne
09.2017 - 08.2019 Master of Science (M. Sc.) Westphalian University, Recklinghausen, Molecular Biology
Master’s thesis: Purification and characterization of a soluble BMP2 variant of high heparin affinity
Supervisor: Prof. Dr. Angelika Loidl-Stahlhofen - 09.2014-08.2017 Bachelor of Science (B.Sc.) Westphalian University, Recklinghausen, Molecular Biology
Bachelor’s thesis: Establishment and optimization of a new expression and purification strategy for recombinant human paraoxonase 1 in E. coli
Supervisor: Prof. Dr. Peter Bayer
Research
In the research group of Prof. Jan von Langermann, I study biocatalytic reactions with the focus on biotechnological, synthetic, and process-optimizing approaches including in situ removal of products.
On the one hand, I am working with transaminases (TAs) to produce enantiomerically pure amines in various reactions, conducted by TAs in a soluble or immobilized form. Immobilization is realized with different strategies and allows the application in a repetitive batch or continuously operating flow mode. To maximize product yields, substrates are continuously fed, while the product is continuously removed, e.g. by selective crystallization, which allows a simplified separation. Cascading TAs with an amino acid oxidase and a catalase to artificial enzyme cascades further improves reaction efficiencies.
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On the other hand, products of biocatalytically hydrolyzed synthetic polymers (i.e., plastics) should be selectively separated from the reaction medium. Common plastic models are therefore hydrolyzed by a range of different hydrolases under various conditions, while a particular intermediate should be enforced over other products by reaction engineering. The intermediate will then be extracted in situ by several strategies. Chemical synthesis of different substrate molecules is currently being addressed. Analysis of products is generally realized by spectroscopic, HPLC- and NMR-measurements. If you are looking for a place for an internship or a thesis in the field of biochemistry (biocatalysis) and biotechnology, or if you have any questions, suggestions or just want to get in touch, please do not hesitate to contact me. I am always happy and open to new input and collaborations. |
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Scientific Papers
- Heinks, T.*; Hettwer, A*.; Hiepen, C.; Weise, C.; Gorka, M.; Knaus, P.; Mueller, T.D.; Loidl-Stahlhofen, A. Optimized expression and purification of a soluble BMP2 variant based on in-silico design. Protein Expr. Purif. 2021, 186, 105918, doi:10.1016/j.pep.2021.105918.
- Janson, N.; Heinks, T.; Beuel, T.; Alam, S.; Höhne, M.; Bornscheuer, U.T.; Fischer von Mollard, G.; Sewald, N. Efficient Site‐Selective Immobilization of Aldehyde‐Tagged Peptides and Proteins by Knoevenagel Ligation. ChemCatChem 2022, 14, doi:10.1002/cctc.202101485.
- Heinks, T.; Paulus, J.; Koopmeiners, S.; Beuel, T.; Sewald, N.; Höhne, M.; Bornscheuer, U.T.; Fischer von Mollard, G. Recombinant L-Amino Acid Oxidase with Broad Substrate Spectrum for Co-substrate Recycling in (S)-Selective Transaminase-Catalyzed Kinetic Resolutions. ChemBioChem 2022, 23, e202200329, doi:10.1002/cbic.202200329.
- Heinks, T.; Montua, N.; Teune, M.; Liedtke, J.; Höhne, M.; Bornscheuer, U.T.; Fischer von Mollard, G. Comparison of Four Immobilization Methods for Different Transaminases. Catalysts 2023, 13, 300, doi:10.3390/catal13020300.
- Heinks, T.; Montua, N.; Teune, M.; Liedtke, J.; Höhne, M.; Bornscheuer, U.T.; Fischer von Mollard, G. Data for 'Comparison of Four Immobilization Methods for Different Transaminases', Bielefeld University PUB-Repository 2023, doi:10.4119/unibi/2968396
- Heinks, T.*; Merz, L.M.*; Liedtke, J.; Höhne, M.; van Langen, L.M.; Bornscheuer, U.T.; Fischer von Mollard, G.; Berglund, P. Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases. Catalysts 2023, 13, 875, doi:10.3390/catal13050875.
- Heinks, T.; Koopmeiners, S.*; Montua, N.*; Sewald, N.; Höhne, M.; Bornscheuer, U.T.; Fischer von Mollard, G. Co-Immobilization of a Multi-Enzyme Cascade: (S)-Selective Amine Transaminases, L-Amino Acid Oxidase and a Catalase. ChemBioChem 2023, e202300425, doi:10.1002/cbic.202300425.
*contributed equally to work
Conference Contributions
- 03.2023 Novel Enzymes 2023
Poster: Cascading of (S)-Selective Transaminases with an L-Amino Acid Oxidase and a Catalase - 03.2022 10th International Congress on Biocatalysis (BioCat2022)
Poster: Artificial Enzyme Cascade for Kinetic Resolutions with Co-Substrate Recycling of (S)-Selective Transaminases - 04.2022 Joint Seminar Chemistry and Biochemistry
Presentation: Establishment of artificial and immobilized enzyme cascades involving transaminases and L-amino acid oxidase for kinetic resolution of racemic amines - 03.2022 Intensifying Biocatalytic Processes: From Enzyme and Material Design to Industrial Applications (IBPRO2022) Poster: Recycling of Co-Substrates in Transaminase-Catalyzed Kinetic Resolutions
- 11.2020 Graduate School of Chemistry and Biochemistry
Presentation: Establishment of artificial and immobilized transaminase involved enzyme cascades for derazemization of amino acid racemates - 02.2018/19 Bioprocessing Days 2018/19
